Human CD8+ T Cell Responses to EBV EBNA1: HLA Class I Presentation of the (Gly-Ala)–Containing Protein Requires Exogenous Processing
Identifieur interne : 003D60 ( Main/Exploration ); précédent : 003D59; suivant : 003D61Human CD8+ T Cell Responses to EBV EBNA1: HLA Class I Presentation of the (Gly-Ala)–Containing Protein Requires Exogenous Processing
Auteurs : Neil Blake [Royaume-Uni] ; Steven Lee [Royaume-Uni] ; Irina Redchenko [Royaume-Uni] ; Wendy Thomas [Royaume-Uni] ; Neil Steven [Royaume-Uni] ; Alison Leese [Royaume-Uni] ; Patty Steigerwald-Mullen [États-Unis] ; Michael G. Kurilla [États-Unis] ; Lori Frappier [Canada] ; Alan Rickinson [Royaume-Uni]Source :
- Immunity [ 1074-7613 ] ; 1997.
English descriptors
- Teeft :
- Acad, Adenovirus, Antigen processing, Assay, Autologous, Baculovirus, Cell clones, Cell line, Cell response, Cell responses, Chromium, Chromium release assay, Chromium release assays, Clone, Control targets, Control vaccinia, Ctls, Cytotoxic, Dmso, E1cd, Ebna1, Ebna1 coding sequence, Ebna1 epitope, Ebna1 epitope peptide, Ebna1 epitopes, Ebna1 fragments, Ebna1 protein, Ebna1 sequence, Endogenously, Endoplasmic reticulum, Epitope, Epitope ebna1, Exogenous, Exogenous antigen, Exogenously, Fusion protein, Growth medium, Healthy donors, Histocompatibility, Human cytomegalovirus, Immunol, Khanna, Kieff, Kurilla, Latent cycle proteins, Levitskaya, Lymphocyte, Lysis, Major histocompatibility, Minimal epitope, Molecular weight, Natl, Npc4, Nuclear antigen, Pathway, Peptide, Peripheral blood lymphocytes, Plasmid, Preexposed, Preincubated, Proc, Protein extracts, Recent work, Recombinant, Recombinant adenoviruses, Recombinant vaccinia vectors, Recombinant vaccinia viruses, Representative results, Rickinson, Sensitization, Smaller peptides, Solid bars, Standard chromium release assay, Steven, Striped bars, Synthetic epitope peptide, Synthetic peptides, Target cells, Unpublished data, Vaccinia, Viral, Virol.
Abstract
Abstract: Epstein-Barr virus (EBV)–induced cytotoxic T lymphocyte (CTL) responses have been detected against many EBV antigens but not the nuclear antigen EBNA1; this has been attributed to the presence of a glycine-alanine repeat (GAr) domain in the protein. Here we describe the isolation of human CD8+ CTL clones recognizing EBNA1-specific peptides in the context of HLA-B35.01 and HLA-A2.03. Using these clones, we show that full-length EBNA1 is not presented when expressed endogenously in target cells, whereas the GAr-deleted form is presented efficiently. However, when supplied as an exogenous antigen, the full-length protein can be presented on HLA class I molecules by a TAP-independent pathway; this may explain how EBNA1-specific CTLs are primed in vivo.
Url:
DOI: 10.1016/S1074-7613(00)80397-0
Affiliations:
- Canada, Royaume-Uni, États-Unis
- Angleterre, Midlands de l'Ouest, Ontario
- Birmingham, Toronto
- Université de Birmingham, Université de Toronto
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Here we describe the isolation of human CD8+ CTL clones recognizing EBNA1-specific peptides in the context of HLA-B35.01 and HLA-A2.03. Using these clones, we show that full-length EBNA1 is not presented when expressed endogenously in target cells, whereas the GAr-deleted form is presented efficiently. However, when supplied as an exogenous antigen, the full-length protein can be presented on HLA class I molecules by a TAP-independent pathway; this may explain how EBNA1-specific CTLs are primed in vivo.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Midlands de l'Ouest</li><li>Ontario</li></region><settlement><li>Birmingham</li><li>Toronto</li></settlement><orgName><li>Université de Birmingham</li><li>Université de Toronto</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Blake, Neil" sort="Blake, Neil" uniqKey="Blake N" first="Neil" last="Blake">Neil Blake</name></region><name sortKey="Lee, Steven" sort="Lee, Steven" uniqKey="Lee S" first="Steven" last="Lee">Steven Lee</name><name sortKey="Leese, Alison" sort="Leese, Alison" uniqKey="Leese A" first="Alison" last="Leese">Alison Leese</name><name sortKey="Redchenko, Irina" sort="Redchenko, Irina" uniqKey="Redchenko I" first="Irina" last="Redchenko">Irina Redchenko</name><name sortKey="Rickinson, Alan" sort="Rickinson, Alan" uniqKey="Rickinson A" first="Alan" last="Rickinson">Alan Rickinson</name><name sortKey="Rickinson, Alan" sort="Rickinson, Alan" uniqKey="Rickinson A" first="Alan" last="Rickinson">Alan Rickinson</name><name sortKey="Steven, Neil" sort="Steven, Neil" uniqKey="Steven N" first="Neil" last="Steven">Neil Steven</name><name sortKey="Thomas, Wendy" sort="Thomas, Wendy" uniqKey="Thomas W" first="Wendy" last="Thomas">Wendy Thomas</name></country><country name="États-Unis"><noRegion><name sortKey="Steigerwald Mullen, Patty" sort="Steigerwald Mullen, Patty" uniqKey="Steigerwald Mullen P" first="Patty" last="Steigerwald-Mullen">Patty Steigerwald-Mullen</name></noRegion><name sortKey="Kurilla, Michael G" sort="Kurilla, Michael G" uniqKey="Kurilla M" first="Michael G" last="Kurilla">Michael G. Kurilla</name></country><country name="Canada"><region name="Ontario"><name sortKey="Frappier, Lori" sort="Frappier, Lori" uniqKey="Frappier L" first="Lori" last="Frappier">Lori Frappier</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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